LAMBDA 365+/465 UV/Vis Spectrophotometer
Model: LAMBDA 365+ / 465The workhorse UV/Vis for universities and QA labs: 190 – 1100 nm. The 365+ uses a double-beam Czerny-Turner optical system with 0.5 – 20 nm variable bandwidth and 4 A absorbance; the 465 uses a photodiode array that captures a full spectrum in 20 ms. A large compartment takes over ten sampling accessories including autosamplers and thermostatted cell holders, with 21 CFR Part 11 compliant software.
Specifications
| Models | LAMBDA 365+ (double beam) / LAMBDA 465 (PDA) |
|---|---|
| Wavelength range | 190 – 1100 nm |
| Spectral bandwidth | 365+: 0.5 / 1 / 2 / 5 / 20 nm variable; 465: 1 nm |
| Optical system | 365+: double-beam Czerny-Turner; 465: single-beam photodiode array |
| Photometric range | up to 4 A |
| Wavelength accuracy | ±0.1 nm at 656.1 nm; ±0.3 nm across range |
| Wavelength reproducibility | better than 0.05 nm |
| Scan speed | 365+: 7.5 – 12000 nm/min; 465: full spectrum in 20 ms |
| Stray light | 465: < 0.03 %T at 340 nm |
| Light sources | Pre-aligned deuterium + tungsten-halogen, automatic switching |
| Sample compartment | Large compartment, more than 10 accessories supported |
| Software | UV WinLab, 21 CFR Part 11 compliant |
| Dimensions | 595 × 272 × 535 mm, 23.8 kg (365+) |
Features
- 190 – 1100 nm covers the full UV/Vis range for quantitation, kinetics and purity checks
- 365+ double-beam Czerny-Turner design measures sample and reference together, so long kinetic runs do not drift
- 0.5 – 20 nm variable bandwidth balances high resolution against throughput
- 4 A photometric range takes concentrated samples without dilution
- 365+ scans at 7.5 – 12000 nm/min to capture fast reaction kinetics
- 465 photodiode array acquires a complete spectrum in 20 ms — ideal for dissolution and fast kinetics
- Large compartment accepts multi-cell changers, thermostatted holders, autosamplers and more
- UV WinLab ships with method templates and performance verification; the ES edition meets 21 CFR Part 11
Applications
- Teaching: spectrophotometry fundamentals, Beer-Lambert verification, DNA and protein quantitation
- Pharma and drug testing: dissolution, content uniformity, related substances, method development
- Food and agriculture: total phenolics, flavonoids, nitrite, pigments and additives
- Environmental monitoring: total phosphorus, total nitrogen, hexavalent chromium, COD
- Life science: enzyme activity, protein concentration, nucleic acid purity (A260/A280)
- Chemicals and materials: reaction kinetics, catalyst activity, in-line concentration monitoring